ZAVY.htm

NR ZAVY

AU Mukasa-Mugerwa,E.; Bekele,T.; Kasali,O.B.

TI The effect of handling methods on subsequent plasma progesterone levels in sheep

QU Veterinary Research Communications 1989; 13(1): 75-9

PT journal article

AB The mean progesterone concentration in the plasma of 10 adult Ethiopian Highland sheep obtained immediately after slaughter was 10.56 +/- 3.98 ng/ml. Samples were subsequently incubated at 4 degrees C, room temperature (19-22 degrees C) or 26 degrees C as either plasma or intact but citrated blood. Failure to separate plasma affected the progesterone content at 2-72 h at room temperature or 26 degrees C (p less than 0.01 - p less than 0.0001). Incubation temperature affected the plasma concentration at 18 h (p less than 0.05) and 24 h (p less than 0.001). Although progesterone values were generally higher in separated plasma, disparity with the values from plasma separated from incubated citrated blood was small (r = 0.76-0.98). Progesterone concentration declined haphazardly after collection but sometimes exceeded the initial readings. This kept the average concentration of progesterone in plasma separated immediately after collection fairly constant and within 15% of zero time samples during the first 48 h.

AD Animal Reproduction and Health Section, International Livestock Centre for Africa, Addis Ababa, Ethiopia.

SP englisch

PO Niederlande

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