NR AAAV
AU Ackerman,S.L.; Knowles,B.B.; Andrews,P.W.
TI Gene regulation during neuronal and non-neuronal differentiation of NTERA2 human teratocarcinoma-derived stem cells
QU Brain Research. Molecular Brain Research 1994 Aug; 25(1-2): 157-62
PT journal article
AB We constructed cDNA libraries from a clonal human teratocarcinoma-derived cell line and two retinoic acid-induced derivatives: a homogeneous population of neurons and a FACS-isolated, non-neuronal population. These libraries are large and representative of the cells from which they were derived, as determined by colony hybridization. PCR analysis indicates that the transcripts encoding P- and E-cadherin are down-regulated whereas the the prion protein (PrP) transcript is up-regulated in neurons. These cells offer a promising system for investigations of human prion infection and the cDNA libraries provide a source of neuron-specific genes.
MH Antibodies, Monoclonal; Base Sequence; Cell Differentiation/drug effects/genetics; Cell Separation; Flow Cytometry; Gene Expression Regulation, Neoplastic/*physiology; Genomic Library; Human; Molecular Sequence Data; Support, U.S. Gov't, P.H.S.; Teratocarcinoma/*genetics/pathology; Tretinoin/pharmacology; Tumor Cells, Cultured; Tumor Stem Cells/*cytology
AD Jackson Laboratory, Bar Harbor, ME 04609.
SP englisch
PO Niederlande