NR AAEC
AU Aiken,J.M.; Williamson,J.L.; Borchardt,L.M.; Marsh,R.F.
TI Presence of mitochondrial D-loop DNA in scrapie-infected brain preparations enriched for the prion protein
QU Journal of Virology 1990 Jul; 64(7): 3265-8
IA http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=1972202
PT journal article
AB The prion preparation has, in recent years, been the focal point of scrapie research. The inability to identify agent-specific nucleic acids in this sample has led to the formulation of the infectious protein or prion hypothesis. In this study, we analyzed three different prion protein-enriched preparations and found all to contain significant amounts of mitochondrial nucleic acid. Southern blot analyses indicated that they are enriched for a specific component of the mitochondrial genome, the single-stranded displacement loop fragment. Our results suggest that if mitochondrial nucleic acids are involved in scrapie infection, it is the displacement loop fragment that is specifically responsible.
MH Animals; Brain Chemistry; Cell Fractionation/methods; Cricetinae; DNA, Mitochondrial/*analysis; PrPsc Proteins; Regulatory Sequences, Nucleic Acid; Research Support, U.S. Gov't, Non-P.H.S.; Scrapie/*transmission; Viral Proteins/*isolation & purification
AD Department of Veterinary Science, University of Wisconsin-Madison 53706.
SP englisch
PO USA