NR AAWZ
AU Baker,H.F.; Ridley,R.M.; Wells,G.A.H.; Ironside,J.W.
TI Prion protein immunohistochemical staining in the brains of monkeys with transmissible spongiform encephalopathy
QU Neuropathology and Applied Neurobiology 1998 Dec; 24(6): 476-86
PT journal article
AB Prion protein (PrP) immunohistochemical staining of the brains of common marmosets (Callithrix jacchus) with experimental transmissible spongiform encephalopathy is described. The monkeys (n = 17) had been injected, intracerebrally, 17-49 months previously with homogenates of brain tissue taken post mortem from a cow with BSE (n = 2 monkeys), a sheep with natural scrapie (n = 2 monkeys), human cases of growth hormone related Creutzfeldt-Jakob disease (CJD) (n = 2 monkeys), sporadic CJD (n = 5 monkeys), or Gerstmann-Sträussler-Scheinker disease (GSS) (n = 4 monkeys), or from monkeys with spongiform encephalopathy resulting from injection with brain tissue from these last two cases (n = 1 monkey from each case). Only diffuse PrP-staining was seen in monkeys injected with CJD-material whereas more aggregated deposits of PrP were seen in monkeys injected with BSE-, scrapie - and GSS-brain tissue. There were no patterns of staining specific to the brains injected with BSE-material that could be used to identify the origin of that inoculum. BSE - and scrapie-injected monkey brains could be distinguished from each other because in BSE-injected monkey brain the spongiform vacuolation was largely confined to subcortical structures whereas in scrapie-injected monkey brain the spongiform vacuolation was also prominent in the neocortex. The patterns of PrP deposition differed markedly between those seen in monkey brains injected with BSE-material or CJD-material, but the patterns of PrP staining seen in monkey brains injected with BSE-material were also seen in monkey brains injected with scrapie - or GSS-material. Overall there was a correlation between the length of the incubation period and the amount of aggregated PrP-staining, but no correlation between the neuropathological picture and the clinical presentation of neurological signs.
MH Adult; Aged; Animal; Brain Chemistry/*physiology; Callithrix; Case Report; Cattle; Female; Human; Immunohistochemistry; Middle Age; Nerve Tissue Proteins/*analysis; Prion Diseases/*metabolism; Prions/*analysis; Sheep; Staining and Labeling; Vacuoles/pathology
AD Department of Neuropathology, Central Veterinary Laboratory, New Haw, UK
SP englisch
PO England