NR AAXR
AU Baldwin,M.A.; Stahl,N.; Reinders,L.G.; Gibson,B.W.; Prusiner,S.B.; Burlingame,A.L.
TI Permethylation and tandem mass spectrometry of oligosaccharides having free hexosamine: analysis of the glycoinositol phospholipid anchor glycan from the scrapie prion protein.
QU Analytical Biochemistry 1990 Nov 15; 191(1): 174-82
PT journal article
AB Permethylation of the glycan isolated from the glycoinositol phospholipid (GPI) anchor of the scrapie prion protein (PrPsc) trimethylates a free hexosamine to form a quarternary ammonium salt, substantially increasing the sensitivity for analysis by mass spectrometry. This derivatization induces specific fragmentation reactions in collision-induced dissociation spectra obtained on a four-sector tandem mass spectrometer, identifying the branching pattern of the PrPsc GPI glycan.
MH Glycolipids/*analysis; Glycosylphosphatidylinositols; Hexosamines/*chemistry; Methylation; Molecular Structure; Oligosaccharides/*chemistry; Phosphatidylinositols/*analysis; PrPsc Proteins; Prions/*analysis; Spectrum Analysis; Support, U.S. Gov't, Non-P.H.S.; Support, U.S. Gov't, P.H.S.; Viral Proteins/*analysis/chemistry
AD Department of Pharmaceutical Chemistry, University of California, San Francisco 94143.
SP englisch
PO USA