NR ABCX
AU Beaudry,P.; Cohen,P.; Brandel,J.P.; Delasnerie-Laupretre,N.; Richard,S.; Launay,J.M.; Laplanche,J.L.
TI 14-3-3 protein, neuron-specific enolase, and S-100 protein in cerebrospinal fluid of patients with Creutzfeldt-Jakob disease
QU Dementia and Geriatric Cognitive Disorders 1999 Jan-Feb; 10(1): 40-6
PT clinical trial; journal article
AB We explored simultaneously 14-3-3 protein, neuron-specific enolase (NSE), and one astroglial protein, S-100, recently proposed as Creutzfeldt-Jakob disease (CJD) markers, in the cerebrospinal fluid (CSF) of 129 patients with suspected CJD. Cutoff values for NSE and S-100 were established at 25 and 2.5 ng/ml, respectively. The highest sensitivity was observed for S-100 (94.2%) followed by 14-3-3 (89.8%) and NSE (79.7%), while the highest specificity in CJD diagnosis was obtained with 14-3-3 protein (100%) as compared with NSE (91.5%) and S-100 (85.4%). No influence of sex, genotype at codon 129 of the prion protein gene, time between sampling, and death or disease duration has been found. Based on 90 cases initially referred as 'probable' or 'possible' CJD, with 14-3-3, NSE, or S-100 we could correctly discriminate between 'CJD' or 'non-CJD' categories in 94.4, 86.5, and 90% of the cases, respectively. When limited to 'possible CJD' cases, diagnosis based on one of the three CSF proteins was accurate in 98, 90.7 and 87.3%, respectively. In view of the fact that the CSF 14-3-3 protein test alone has the highest specificity and good sensitivity, it appears that there is no additional advantage at the moment to include NSE and/or S-100 protein in the exploration of clinically suspected CJD cases.
IN Die Autoren untersuchten bei 129 Patienten mit dem Verdacht auf Creutzfeldt-Jakob-Krankheit in der Rückenmarksflüssigkeit die Konzentrationen der CJD-Marker 14-3-3, neuronenspezifische Enolase und das S100-Protein. Als Grenzwerte wählten sie 25 ng/ml für die neuronenspezifische Enolase und 2,5 ng/ml für das S100-Protein. Damit erreichten sie Sensitivitäten von 94,2% mit dem S100-Protein, 89,8% mit dem 14-3-3-Test und 79,7% mit der neuronenspezifischen Enolase. Die Spezifitäten lagen bei 100% mit dem 14-3-3-Test 91,5% mit der neuronenspezifischen Enolase und 85,4% mit dem S100-Protein. Die Autoren konnten keinen Einfluß des Geschlechtes, des Genotypes am Codon 129, der Zeitdauer zwischen Probenentnahme und dem Tod, oder der Krankheitsdauer feststellen.
ZR 29
MH Adult; Creutzfeldt-Jakob Syndrome/*cerebrospinal fluid; Female; Human; Immunochemistry; Male; Phosphopyruvate Hydratase/*cerebrospinal fluid; Predictive Value of Tests; Proteins/*metabolism; S100 Proteins/*cerebrospinal fluid; Support, Non-U.S. Gov't
AD Centre de Recherche C. Bernard, IRF 6, Service de Biochimie, Hopital Lariboisiere, Paris Cedex 10, France.
SP englisch
PO Schweiz