NR ABDY
AU Beekes,M.; McBride,P.A.; Baldauf,E.
TI Cerebral targeting indicates vagal spread of infection in hamsters fed with scrapie
QU Journal of General Virology 1998 Mar; 79(3): 601-7
IA http://vir.sgmjournals.org/cgi/reprint/79/3/601
PT journal article
AB The pathogenesis of scrapie and other transmissible spongiform encephalopathies (TSEs) following oral uptake of agent is still poorly understood and can best be studied in mice and hamsters. The experiments described here further extend the understanding of the pathways along which infection spreads from the periphery to the brain after an oral challenge with scrapie. Using TSE-specific amyloid protein (TSE-AP, also called PrP) as a marker for infectivity, immunohistochemical evidence suggested that the first target area in the brain of hamsters orally infected with scrapie is the dorsal motor nucleus of the vagus nerve (DMNV), rapidly followed by the commissural solitary tract nucleus (SN). The cervical spinal cord was affected only after TSE-AP had been deposited in the DMNV, SN and other medullary target areas. For the first time, these results demonstrate conclusively that, in our animal model, initial infection of the brain after oral ingestion of scrapie agent occurs via the vagus nerve, rather than by spread along the spinal cord.
ZR 20 Zitate
IN Hamster wurden oral mit Hamsterscrapie infiziert. Im Gehirn wurde daraufhin das proteaseresistente Prionprotein immunohistochemisch zuerst am Ende des Vagusnerven im motorischen Nucleus dorsalis nervi vagi im Bereich der Rautengrube. Kurz darauf wurde das proteaseresistente Prionprotein in von dort weiterführenden Nervenfasern markiert. Erst später wurde das Amyloid im Rückenmark gefunden. Zumindest bei oral infizierten Hamstern scheint also der Erreger über den Vagusnerven direkt vom Magen in das Gehirn zu wandern.
MH Administration, Oral; Animals; Biological Markers; Brain/*metabolism; Cricetinae; Immunohistochemistry; Prions/metabolism/*pathogenicity; Research Support, Non-U.S. Gov't; Scrapie/*etiology; Solitary Nucleus/metabolism; Spinal Cord/metabolism; Time Factors; Vagus Nerve/*metabolism
AD Michael Beekes (BeekesM@rki.de), Robert Koch-Institut, Bundesinstitut für Infektionskrankheiten und nichtübertragbare Krankheiten, Berlin, Germany.; Patricia A. McBride (tricia.mcbride@bbsrc.ac.uk), Institute for Animal Health, BBSRC and MRC, Neuropathogenesis Unit, Ogston Building, West Mains Road, Edinburgh EH9 3JF, Scotland, UK
SP englisch
PO England