NR ABFL
AU Bencsik,A.A.; Lezmi,S.; Baron,T.G.M.
TI Autonomic nervous system innervation of lymphoid territories in spleen: a possible involvement of noradrenergic neurons for prion neuroinvasion in natural scrapie.
QU Journal of Neurovirology 2001 Oct; 7(5): 447-53
PT journal article
AB In experimental as well as in natural scrapie, transmissible spongiform encephalopathies (TSEs), the infectious agent, closely related to PrPsc, an abnormal isoform of the cellular prion protein, invades and replicates in lymphoid organs such as spleen before affecting the brain. To clarify the cellular requirements for the possible neuroinvasion of scrapie agent from the spleen to the central nervous system, we have studied the sympathetic innervation within the lymphoid territories of the spleen. These noradrenergic fibers originating from the coeliomesenteric ganglia were examined with regard to PrPsc-associated cells in spleen of adult sheep severely affected with natural scrapie. Using a double immunolabelling strategy, we demonstrated the proximity of noradrenergic endings with PrPsc-accumulating cells, strengthening its possible implication in the neuroinvasion process.
MH Adrenergic Fibers/chemistry/*pathology; Animal; Antibodies, Monoclonal/immunology; Axonal Transport; Central Nervous System/chemistry/*pathology; Cerebellum/chemistry/pathology; Ganglia, Sympathetic/chemistry/pathology; Genetic Predisposition to Disease; Immunoenzyme Techniques; Posterior Horn Cells/chemistry/pathology; PrPsc Proteins/immunology/*isolation & purification; Scrapie/genetics/*pathology; Sheep; Spleen/*innervation/pathology; Support, Non-U.S. Gov't; Sympathetic Nervous System/chemistry/*pathology
AD Anna A. Bencsik (a.bencsik@lyon.afssa.fr), Stéphane Lezmi (s.lezmi@lyon.afssa.fr), Thierry G. M. Baron (t.baron@lyon.afssa.fr), Unité Agents Transmissibles Non Conventionnels (ATNC), Agence Francaise de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, 31 Avenue Tony Garnier, 69364 Lyon cedex 07, France
SP englisch
PO USA