NR ABGH
AU Beranger,F.; Mange,A.; Solassol,J.; Lehmann,S.
TI Cell culture models of transmissible spongiform encephalopathies
QU Biochemical and Biophysical Research Communications 2001 Nov 30; 289(2): 311-6
PT journal article; review; review, tutorial
AB In this review, we describe the generation and use of cell culture models of transmissible spongiform encephalopathies, also known as prion diseases. These models include chronically prion-infected cell lines, as well as cultures expressing variable amounts of wild-type, mutated, or chimeric prion proteins. These cell lines have been widely used to investigate the biology of both the normal and the pathological isoform of the prion protein. They have also contributed to the comprehension of the pathogenic processes occurring in transmissible spongiform encephalopathies and in the development of new therapeutic approaches of these diseases.
ZR 59
MH Animal; Blotting, Western; *Cells, Cultured; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Human; Models, Biological; Mutation; Phosphoprotein Phosphatase/metabolism; PrPsc Proteins/metabolism; Prion Diseases/*metabolism/*pathology/*therapy; Prions/*metabolism; Recombinant Fusion Proteins/metabolism; Support, Non-U.S. Gov't
AD Institut de Genetique Humaine, CNRS UPR 1142, 141, rue de la Cardonille, 34396 Montpellier Cedex 5, France.
SP englisch
PO USA