NR ABIB
AU Betmouni,S.; Perry,V.H.
TI The acute inflammatory response in CNS following injection of prion brain homogenate or normal brain homogenate
QU Neuropathology and Applied Neurobiology 1999 Feb; 25(1): 20-8
PT journal article
AB The neuropathological hallmarks of end-stage prion disease are vacuolation, neuronal loss, astrocytosis and deposition of PrPsc amyloid. We have also shown that there is an inflammatory response in the brains of scrapie-affected mice from 8 weeks post-injection. In this study we have investigated the acute CNS response to the intracerebral injection of scrapie-affected brain homogenate. The ME7 strain of scrapie (Neuropathogenesis Unit, Edinburgh) was used, and control mice were injected with brain homogenate derived from normal C57BL/6 J mice. One microlitre of 10% w/v ME7 (n = 33) and normal brain homogenate (n = 28) was injected stereotaxically into the right dorsal hippocampus. Cryostat sections of brains taken at 1, 2, 5, 7, 14 and 28 days post-injection were examined histologically for neuronal loss, and immunocytochemically to study the inflammatory response. This study shows that ME7 is not acutely neurotoxic in vivo. There is also no difference (ANOVA) in the inflammatory response, which peaked between 2 and 5 days and resolved by 4 weeks after intracerebral injection of either ME7 or normal brain homogenate. The well circumscribed inflammatory response seen previously at 8 weeks is therefore a consequence of a disease process rather than a surgical artefact. This disease process may be related to a localized accumulation of PrPsc sufficient to stimulate an inflammatory response which in turn may contribute to neuronal loss. The role of the inflammatory response in chronic neurodegeneration can be usefully studied using this mouse model of prion disease, and this will undoubtedly shed light on the pathogenic mechanisms underlying other chronic neurodegenerative diseases.
IN Mäusen wurde ein Mikroliter einer 10%igen Gehirnlösung entweder von mit dem Scrapiestamm ME7 infizierten oder von normalen Mäusen in den rechten dorsalen Hippocampus. 1, 2, 5, 7, 14 oder 28 Tage nach der Injektion wurden den Mäusen Hirnproben entnommen und histologisch und immunocytochemisch untersucht. ME7-infektiöses Gehirn erwies sich als nicht akut neurotoxisch. Die Inokulationen mit normalen ebenso wie die mit infektiösen Hirnsuspensionen führten zu Entzündungsreaktionen mit Maxima nach 2-5 Tagen, die aber nach 4 Wochen abgeklungen waren.
ZR 37
MH Acute Disease; Animal; Brain Diseases/*etiology/immunology/pathology; Female; Immunohistochemistry; Male; Mice; Mice, Inbred C57BL; Microglia/pathology; Neuritis/*etiology/immunology/pathology; Neurons/pathology; Prion Diseases/*complications/immunology/pathology; Support, Non-U.S. Gov't; T-Lymphocytes/immunology
AD School of Biological Sciences, University of Southampton, UK
SP englisch
PO England