NR ABII
AU Biernat,W.; Liberski,P.P.; Guiroy,D.C.; Yanagihara,R.; Gajdusek,D.C.
TI Proliferating cell nuclear antigen immunohistochemistry in astrocytes in experimental Creutzfeldt-Jakob disease and in human kuru, Creutzfeldt-Jakob disease and Gerstmann-Sträussler-Scheinker syndrome
QU Neurodegeneration. A Journal for neurodegenerative Disorders, Neuroprotection, and Neuroregeneration 1995 Jun; 4(2): 195-201
PT journal article
AB We have used immunohistochemical techniques and a monoclonal antibody against proliferating cell nuclear antigen (PCNA) to investigate the proliferative activity of glial cells in mice with experimental Creutzfeldt-Jakob disease (CJD), and in human cases of CJD, kuru and Gerstmann-Sträussler-Scheinker syndrome (GSS). Only a small proportion of hypertrophic astrocytes showed PCNA immunoreactivity (labelling index, LI: 0-4.5%). PCNA-specific immunostaining was confined entirely to cell nuclei. During the early stages of illness, with minimal CJD pathology, PCNA-immunopositive nuclei were occasionally observed in the subependymal zone of experimentally infected mice. From 18 weeks postinoculation, PCNA-immunopositive astrocytes were most frequently found in the corpus callosum and cerebellar white matter; regions which characteristically exhibit robust vacuolation. No other cells, particularly no cells of microglial morphology, showed PCNA immunoreactivity. In human cases of kuru, CJD and GSS, no PCNA-immunopositive cells were detected despite the presence of numerous microglial cells and reactive hypertrophic astrocytes. These results indicate that only a limited proportion of astrocytes proliferate in the experimental models of subacute spongiform encephalopathies and that microglia are probably postmitotic cells.
IN Bei Menschen und Tieren mit übertragbaren spongiformen Enzephalopathien wurde festgestellt, dass nur ein kleiner Teil der Astrozyten sich teilte.
ZR 48
MH Animal; Astrocytes/*immunology; Creutzfeldt-Jakob Syndrome/etiology/*immunology/pathology; Gerstmann-Sträussler-Scheinker Disease/*immunology/pathology; Human; Immunoenzyme Techniques; Kuru/*immunology/pathology; Mice; Microglia/*immunology; Proliferating Cell Nuclear Antigen/*analysis; Support, Non-U.S. Gov't
AD Laboratory of Tumor Biology, Medical Academy Lodz, Poland.
SP englisch
PO England