NR ABMY
AU Borkhsenius,A.S.; Sasnauskas,K.; Gedvilaite,A.; Inge-Vechtomov,S.G.
TI [Chimeric yeast prions with unstable inheritance]
OT Khimernye priony drozhzhei s nestabil'nym nasledovaniem
QU Genetika 2002 Mar; 38(3): 300-5
PT journal article
AB The Saccharomyces cerevisiae [PSI] factor, a cytoplasmic omnipotent nonsense suppressor, is a conformationally changed (prion) form of translation termination factor eRF3 (Sup35p). Induction and maintenance of the [PSI] factor depend on the prionizing peptide located in the N domain of Sup35p. The N domain of Sup35p was fused with phosphoribosylaminoimidazole carboxylase (Ade2p), a purine biosynthesis enzyme, and the hybrid protein (NM-Sup35p::Ade2p) was tested for induction of the [PSI] factor. Transformation with a centromeric plasmid carrying the gene for NM-Sup35p::Ade2p induced a [PSI]-like factor in yeast cells, which was evident from efficient nonsense suppression. The suppressory effect depended on the presence of the prionizing peptide both in the hybrid protein and in Sup35p synthesized from the chromosomal gene, as well as on the presence of the prion-like [PIN] factor in the cell.
MH Carboxy-Lyases/*genetics/metabolism; English Abstract; Fungal Proteins/*genetics/metabolism; Genes, Suppressor; Prions/*genetics/metabolism; Recombinant Proteins/genetics/metabolism; Saccharomyces cerevisiae/*genetics; Transformation, Genetic
AD Department of Genetics and Breeding, St. Petersburg State University, St. Petersburg, 119034 Russia.
SP russisch
PO Russland