NR ABNA
AU Borras,M.T.; Kingsbury,D.T.; Gajdusek,D.C.; Gibbs,C.J.Jr.
TI Inability to transmit scrapie by transfection of mouse embryo cells in vitro
QU Journal of General Virology 1982 Feb; 58(2): 263-71
IA http://vir.sgmjournals.org/cgi/reprint/58/2/263
PT journal article
AB Infectivity of nucleic acid from highly infectious mouse scrapie brains was studied by transfecting the nucleic acid in vitro prior to inoculation into animals. Foetal mouse brain and whole mouse embryo cultures were chosen as the cells used for the transfection. As an internal control, infectious nucleic acid was recovered from bacteriophage phi X174 and whole virus particles were obtained when cultures were transfected with herpes simplex virus DNA. In contrast, none of the animals inoculated with nucleic acid preparations derived from scrapie-infected tissues developed scrapie disease either with or without transfection procedures. These findings (i) show transfection techniques fail to elicit evidence of a scrapie-specific infectious nucleic acid and (ii) confirm the observation that scrapie is not a viroid.
MH Animal; Brain; Cells, Cultured; DNA, Viral/isolation & purification; Embryo; Mice; Prions/*genetics; Scrapie/*transmission; Sheep; Simplexvirus/genetics; *Transfection
AD Maria-Teresa Borrás, David T. Kingsbury (Present address:Department of Microbiology, School of Medicine, University of California, Irvine, California 92717, USA), D. Carleton Gajdusek, Clarence J. Gibbs, Jr., Laboratory of Central Nervous System Studies, National Institute of Neurological and Communicative Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20205, USA
SP englisch
PO England