NR ABSY
AU Brown,D.R.; Schmidt,B.; Kretzschmar,H.A.
TI Effects of copper on survival of prion protein knockout neurons and glia
QU Journal of Neurochemistry 1998 Apr; 70(4): 1686-93
PT journal article
AB The N-terminal region of the prion protein (PrP) contains an octameric repeat region suggested to bind copper. A 32-amino acid peptide (PrPOcta) based on this region in the protein was tested for its effects on cultured cerebellar cells. Cerebellar cells from mice deficient in cellular PrP (Prnp0/0 mice) are more sensitive to copper toxicity and oxidative stress. PrPOcta selectively promotes the survival of Prnp0/0 cerebellar cells. However, PrPOcta also reduces the toxicity of CuSO4 on cerebellar cells and abolishes the difference in increased sensitivity of Prnp0/0 cells to both copper toxicity and also oxidative stress from xanthine oxidase. PrPOcta does not promote the survival or proliferation of astrocytes or microglia. The survival-promoting effects of PrPOcta on neurons may be due to its ability to effectively chelate copper. The octameric repeat region of PrP may represent a functional domain of the native protein.
MH Animal; Cell Survival/drug effects; Cells, Cultured; Cerebellum/cytology/drug effects; Copper/*pharmacology/poisoning; Mice; Mice, Knockout/*genetics; Neuroglia/*drug effects/physiology; Neurons/*drug effects/physiology; Peptide Fragments/pharmacology; Prions/*genetics/pharmacology; Support, Non-U.S. Gov't
AD Institut für Neuropathologie, Biochemie II, Universität Göttingen, Germany.
SP englisch
PO USA