NR ABTF
AU Brown,D.R.; Schmidt,B.; Kretzschmar,H.A.
TI A neurotoxic prion protein fragment enhances proliferation of microglia but not astrocytes in culture
QU Glia 1996 Sep; 18(1): 59-67
PT journal article
AB The scrapie isoform of the prion protein (PrPsc) induces pathological changes in the central nervous system including neurodegeneration and gliosis. A synthetic prion protein (PrP) peptide corresponding to amino acid residues 106-126 has been shown to be toxic to neurons that express PrPc, the cellular isoform of PrP. Here we show that in mixed glial cultures PrP106-126 induces astroglial proliferation that is dependent on cellular PrPc expression. In purified cultures of glial subtypes only microglia proliferated in response to PrP106-126. This effect was independent of PrP expression. Destruction of microglia in mixed glial cultures by L-leucine methyl ester (LLME) treatment abolished enhanced proliferation caused by PrP106-126. This proliferative effect can be restored by co-culturing LLME-treated astrocytes with microglia. Microglia therefore seem to mediate the proliferative effect exerted by PrP106-126 on astrocytes.
IN Den Aminosäuren 106-126 des menschlichen Prionproteins entsprechende synthetische Peptide bewirkten in Zellkulturen nur in Anwesenheit von Mikrogliazellen eine Astrozytenvermehrung.
ZR 45
MH Amino Acid Sequence; Animal; Astrocytes/*drug effects; Blotting, Western; Cell Division/drug effects; Cells, Cultured; Glial Fibrillary Acidic Protein/biosynthesis; Mice; Microglia/*drug effects; Molecular Sequence Data; Neurotoxins/*pharmacology; Peptides/*pharmacology; PrPsc Proteins/*pharmacology; Stimulation, Chemical; Support, Non-U.S. Gov't
AD Institut für Neuropathologie and Abteilung Biochemie II, Universität Göttingen, Germany.
SP englisch
PO USA