NR ABTU
AU Brown,P.
TI Creutzfeldt-Jakob disease: blood infectivity and screening tests.
QU Seminars in Hematology 2001 Oct; 38(4 Suppl 9): 2-6
PT journal article; review; review, tutorial
AB Blood infectivity data from rodent models of transmissible spongiform encephalopathies (TSEs), and infectivity-prion protein (PrP) equivalence data from scrapie-infected hamster brain suggest a minimum requirement for PrP detection in blood buffy coat of < or = 10 pg/mL. This estimate could either be more or less stringent than calculated-more, if infectivity levels in human blood are lower than in experimental rodent models; less, if the infectivity-PrP ratio is lower in blood than in brain tissue, or if there is a large as yet undetected pool of abnormal but proteinase-sensitive PrP. None of several testing methods under development has yet achieved the calculated sensitivity requirement, but a few are within range, and it should be possible within the coming year to determine whether PrP occurs at a practically detectable level in the blood of patients with TSE.
ZR 21
MH Animal; Creutzfeldt-Jakob Syndrome/*diagnosis/*transmission; Human; Immunoassay/methods/standards; Mass Screening/methods/standards; PrPsc Proteins/blood; Sensitivity and Specificity; Viremia/diagnosis
AD Paul Brown (brownp@ninds.nih.gov), Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892-4122, USA
SP englisch
PO USA