NR ABVP
AU Brown,P.; Liberski,P.P.; Wolff,A.; Gajdusek,D.C.
TI Conservation of infectivity in purified fibrillary extracts of scrapie-infected hamster brain after sequential enzymatic digestion or polyacrylamide gel electrophoresis
QU Proceedings of the National Academy of Sciences of the United States of America 1990 Sep; 87(18): 7240-4
PT journal article
AB Infectious extracts of scrapie-infected hamster brain enriched for scrapie-associated fibrils and scrapie amyloid protein (PrP) were partially denatured and subjected to either polyacrylamide gel electrophoresis with subsequent isolation of the PrP band or sequential enzymatic digestion with deglycosidase, phospholipase, proteinase, and several different nucleases. Infectivity measurements of these various specimens revealed a convincing association between infectivity and scrapie amyloid protein, with or without its sugar chains and disulfide bonds, and did not support the hypothesis that nucleic acid is involved in replication.
IN Hinsichtlich scrapieassoziierter Fibrillen und des Scrapieamyloidproteins (Prionprotein) angereicherte infektiöse Extrakte aus scrapieinfizierten Hamstergehirnen wurden teilweise denaturiert und anschließend einer Polyacrylamidgelelektrophorese unterworfen, oder nacheinander durch die Enzyme Deglycosidase, Phospholipase, Proteinase, sowie durch verschiedene Nukleasen angegriffen. Die Infektivität korrelierte mit der Menge des Prionproteins, unabhängig vom Abspalten von Zuckerketten oder dem Öffnen von Disulfitbrücken. Die Nuklease hatten keinen Einfluß auf die Infektivität.
MH Animal; Brain/*microbiology/pathology; Electrophoresis, Polyacrylamide Gel; Hamsters; Hydrolases; Mesocricetus; Molecular Weight; Prions/*isolation & purification; Scrapie/*microbiology/pathology/transmission
AD Paul Brown (pwb@codon.nih.gov), Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
SP englisch
PO USA
OR Prion-Krankheiten 2 (vermisst)