NR ACGS
AU Carp,R.I.; Merz,G.S.; Licursi,P.C.
TI Scrapie in vitro: agent replication and reduced cell yield.
QU Infection and Immunity 1976 Jul; 14(1): 163-7
PT journal article
AB Exposure of PAM cells, a spontaneously transformed mouse cell line, to brain homogenates from mice infected with scrapie caused a relative decrease in total cell yield, which persisted from passage 2 or 3 to passage 18 after treatment. The effect was elicited by each of the eight independent scrapie isolates tested. Lysates prepared from cultures 16 passages after treatment with scrapie caused the decrease when applied to fresh PAM cultures. Mice inoculated with passage 14 and 18 lysates developed a reduced percentage of polymorphonuclear neutrophils by 5 weeks and scrapie disease by 6 to 9 months after inoculation. Based on the total dilution from treatment of the PAM cultures with scrapie material to the preparation of the lysates, we conclude that the agent(s) responsible for the reduced PAM cell yield, the decreased percentage of polymorphonuclear neutrophils, and the induction of scrapie disease had replicated in the PAM cells. By filtration, the diameter of the agent causing the reduction in cell yield was estimated to be between 25 and 50 nm.
IN Gehirnhomogenate von scrapieinfizierten Mäusen reduzierten die Dichten von Kulturen einer transformierten Mauszelllinie. Dieser Effekt hielt von der 2. oder 3. bis zur 18. Passage an. Nach 16 Passagen haben Lysate dieser Zellkulturen die gleiche Wirkung. Das infektiöse Agens scheint sich in der Zellkultur zu vermehren. Wenn Mäusen Lysate der 14. oder 18. Passage gespritzt wurde, war nach 5 Wochen die Zahl ihrer polymorphonuklearen Neutrophilen reduziert und nach6-9 Monaren entwickelten sie Scrapie. Durch Filtration wurde die Größe des infektiösen Agens auf 25-50 nm geschätzt.
MH Animal; Brain/microbiology; Cell Division; Cell Line; Leukocyte Count; Male; Mice; Neutrophils; Prions/*growth & development/isolation & purification; Rats; Scrapie/blood; Sheep; *Virus Replication
SP englisch
PO USA