NR ACHJ

AU Casaccia-Bonnefil,P.; Kascsak,R.J.; Fersko,R.; Callahan,S.M.; Carp,R.I.

TI Brain regional distribution of prion protein PrP27-30 in mice stereotaxically microinjected with different strains of scrapie

QU Journal of Infectious Diseases 1993 Jan; 167(1): 7-12

PT journal article

AB Stereotaxic inoculation was used to examine the role of scrapie agent strain, inoculum, and injection site on the brain regional distribution of the prion protein, PrP27-30. Neither the type of inoculum nor the injection site influenced the distribution of PrP27-30 in brains of mice. Among the parameters examined, only the strain of agent affected the pattern of distribution and the yield of PrP27-30. Although mice injected into the cerebellum had the shortest incubation period, the cerebellum gave the lowest yield of the PrP27-30 among the seven brain regions examined. The positive correlation between PrP27-30 regional distribution and lesion profile (degree of vacuolation) reinforces the role of the PrPsc protein in scrapie pathogenesis.

IN Nach der Injektion von Scrapie-Prionproteinen in Mausgehirne, entspricht das Ausmaß der Gehirnzerstörung der Verteilung des Prionproteins.

MH Animal; Brain/*metabolism; Mice; Microinjections; PrP 27-30 Protein; Prions/*metabolism; Scrapie/*metabolism; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.

AD New York State Office of Mental Retardation and Developmental Disabilities, Department of Virology, Staten Island.

SP englisch

PO USA

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