NR ACIB
AU Castellani,R.J.; Parchi,P.; Madoff,L.; Gambetti,P.; McKeever,P.
TI Biopsy diagnosis of Creutzfeldt-Jakob disease by western blot: a case report.
QU Human Pathology 1997 May; 28(5): 623-6
PT journal article
AB Creutzfeldt-Jakob disease is a clinically and pathologically heterogeneous disorder that often requires brain biopsy for definitive diagnosis. We report the case of a 62-year-old man who underwent brain biopsy for progressive neurological deterioration. Histopathologically, there was minimal spongiform change that could not be unequivocally attributed to Creutzfeldt-Jakob disease. A 16 mg portion of gray matter saved frozen was subsequently analyzed by Western blot and showed definitive protease-resistant prion protein. This case illustrates applicability, ease in interpretation, and accuracy of Western blot analysis for protease-resistant prion protein in small brain biopsy specimens. Given the importance of accurate diagnosis in suspected prion disease, we recommend that a small portion of tissue from any brain biopsy performed in this setting be kept frozen for possible biochemical studies.
MH Biopsy; Blotting, Western; Brain/metabolism/pathology/ultrastructure; Case Report; Creutzfeldt-Jakob Syndrome/metabolism/*pathology; Fatal Outcome; Human; Immunohistochemistry; Male; Middle Age; Prions/*analysis; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.
AD Department of Pathology, University of Maryland School of Medicine, baltimore, USA
SP englisch
PO USA