NR ACKM
AU Cavallaro,S.; Gibbs,C.J.Jr.; Pergami,P.
TI Early induction of protein nexin-I in scrapie
QU Neuroreport 1999 Jun 3; 10(8): 1677-81
PT journal article
AB We used RNA fingerprinting by arbitrary primed PCR to identify genes whose expression is up-regulated in the brain of hamsters affected by prion disease. One gene implicated by RNA fingerprinting encoded the hamster homologue of protein nexin-I (PN-I), a serine proteinase inhibitor, and was further investigated by Northern blot analysis. PN-I mRNA levels were increased at pre-clinical stages (19 days after inoculation) and remained elevated when the spongiform encephalopathy was anatomopathologically and clinically evident (at 50 and 80 days). Future RNA screening conducted as illustrated may help to reveal a spectrum of genes relevant for the etiopathogenesis and/or diagnosis of prion disease.
MH Animal; Base Sequence; Blotting, Northern; Blotting, Western; Brain/metabolism; Carrier Proteins/*biosynthesis/genetics; Electrophoresis, Polyacrylamide Gel; Female; Hamsters; Immunohistochemistry; Mesocricetus; Molecular Sequence Data; RNA/biosynthesis/genetics; Scrapie/*metabolism; Up-Regulation
AD Istituto di Bioimmagini e Fisiopatologia del Sistema Nervoso Centrale, CNR, Catania, Italy.
SP englisch
PO England