NR ACLQ
AU Chacinska,A.; Boguta,M.; Krzewska,J.; Rospert,S.
TI Prion-dependent switching between respiratory competence and deficiency in the yeast nam9-1 mutant
QU Molecular and Cellular Biology 2000 Oct; 20(19): 7220-9
PT journal article
AB Nam9p is a protein of the mitochondrial ribosome. The respiration-deficient Saccharomyces cerevisiae strain MB43-nam9-1 expresses Nam9-1p containing the point mutation S82L. Respiratory deficiency correlates with a decrease in the steady level of some mitochondrially encoded proteins and the complete lack of mitochondrially encoded cytochrome oxidase subunit 2 (Cox2). De novo synthesis of Cox2 in MB43-nam9-1 is unaffected, indicating that newly synthesized Cox2 is rapidly degraded. Respiratory deficiency of MB43-nam9-1 is overcome by transient overexpression of HSP104, by deletion of HSP104, by transient exposure to guanidine hydrochloride, and by expression of the C-terminal portion of Sup35, indicating an involvement of the yeast prion [PSI(+)]. Respiratory deficiency of MB43-nam9-1 can be reinduced by transfer of cytosol from S. cerevisiae that harbors [PSI(+)]. We conclude that nam9-1 causes respiratory deficiency only in combination with the cytosolic prion [PSI(+)], presenting the first example of a synthetic effect between cytosolic [PSI(+)] and a mutant mitochondrial protein.
MH Cytochrome-c Oxidase/genetics/*metabolism; Cytosol/metabolism; DNA, Fungal/genetics; DNA, Mitochondrial/genetics; Fungal Proteins/chemistry/genetics/*physiology; Gene Deletion; Guanidine/pharmacology; Heat-Shock Proteins/deficiency/genetics/physiology; Mitochondria/physiology; Oxygen Consumption/drug effects; Phenotype; Plant Proteins/genetics/*metabolism; Point Mutation; Prions/genetics/*physiology; Ribosomal Proteins/genetics/*physiology; Saccharomyces cerevisiae/genetics/*metabolism; Support, Non-U.S. Gov't; Translation, Genetic
AD Institute of Biochemistry and Biophysics, 02-106 Warsaw, Poland.
SP englisch
PO USA