NR ACNZ
AU Cherednichenko,Iu.N.; Loginova,N.V.; Mikhailova,G.R.; Vanag,K.A.; Zhdanov,V.M.
TI [Latent infection of continuous L23 cells caused by the agent of scrapie]
OT Latentnaia infektsiia perevivaemykh kletok L23, vyzvannaia agentom skrepi
QU Voprosy Virusologii 1982 Nov-Dec; 27(6): 702-7
PT journal article
AB Two models of latent infection of continuous L23 cells caused by scrapie agent were developed using the method of cell cloning as well as DEAE-dextran and lidase preparations. The infectious activity was found only in cell homogenate L-C and L-506 (cells of clone L23 latently infected with the Compton and C-506 strains of the scrapie agent). A direct relationship between the infectious activity of the cell homogenate and the intensity of brain lesions revealed by morphological examinations of brain sections from infected mice was observed. The cytoplasm of L-C and L-506 cells became strongly vacuolated, piknosis of the nuclei and delay in the cell growth rate were noted. Cytogenetic studies established changes in the model class of the experimental cultures as well as the appearance of rearranged chromosomes.
MH Animal; Cell Line; Cells, Cultured; Chromosome Aberrations; Clone Cells/microbiology; Comparative Study; English Abstract; L Cells (Cell Line)/*microbiology/ultrastructure; Mice; Mice, Inbred BALB C; Prions/*pathogenicity; Time Factors
SP russisch
PO Sowjetunion