NR ACQF
AU Chung,Y.L.; Williams,A.; Chong,A.; Hope,J.; Williams,S.C.R.; Bell,J.D.
TI Metabolic changes associated with vacuolation in murine models of scrapie using in vitro 1H-NMR spectroscopy
QU NMR in Biomedicine 1996 Dec; 9(8): 359-63
PT journal article
AB In this study, metabolic changes in the 79A/C3H, ME7/VM, ME7/C3H, 87V/VM and 22A/SV scrapie mouse models were investigated during the clinical phase of the disease, using in vitro proton nuclear magnetic resonance spectroscopy. N-acetyl-aspartate was found to be significantly reduced in infected mice of the ME7/ C3H (40% reduction), ME7/VM (26%) and 79A/C3H (17%) models when compared to control mice, but not in the 87V/VM and 22A/SV models. The concentration of choline containing compounds and creatine remain unchanged in all models when compared with control murine brains. The level of N-acetyl-aspartate reduction correlated with the extent of grey-matter brain vacuolation. The levels of myo-inositol were found to be significantly increased in the ME7/VM (143%) and 79A/C3H (132%) models only and no significant changes were observed in the ME7/C3H, 87V/VM and 22A/SV models. These changes did not correspond to the severity of gliosis.
MH Animal; Aspartic Acid/analogs & derivatives/metabolism; Brain/*metabolism/pathology; Comparative Study; Hydrogen; Magnetic Resonance Spectroscopy/diagnostic use; Mice; Mice, Inbred C3H; Mice, Inbred Strains; Reference Values; Scrapie/diagnosis/*metabolism; Support, Non-U.S. Gov't; Vacuoles/*pathology/ultrastructure
AD Robert Steiner MR Unit, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK
SP englisch
PO England