NR ACWW
AU Cosson,B.; Couturier,A.; Chabelskaya,S.; Kiktev,D.; Inge-Vechtomov,S.G.; Philippe,M.; Zhouravleva,G.
TI Poly(A)-binding protein acts in translation termination via eukaryotic release factor 3 interaction and does not influence [PSI(+)] propagation
QU Molecular and Cellular Biology 2002 May; 22(10): 3301-15
PT journal article
AB Recent studies of translational control suggest that translation termination may not be simply the end of synthesizing a protein but rather be involved in modulating both the translation efficiency and stability of a given transcript. Using recombinant eukaryotic release factor 3 (eRF3) and cellular extracts, we have shown for Saccharomyces cerevisiae that yeast eRF3 and Pab1p can interact. This interaction, mediated by the N+M domain of eRF3 and amino acids 473 to 577 of Pab1p, was demonstrated to be direct by the two-hybrid approach. We confirmed that a genetic interaction exists between eRF3 and Pab1p and showed that Pab1p overexpression enhances the efficiency of termination in SUP35 (eRF3) mutant and [PSI(+)] cells. This effect requires the interaction of Pab1p with eRF3. These data further strengthen the possibility that Pab1p has a role in coupling translation termination events with initiation of translation. Several lines of evidence indicate that Pab1p does not influence [PSI(+)] propagation. First, "[PSI(+)]-no-more" mutations do not affect eRF3-Pab1p two-hybrid interaction. Second, overexpression of PAB1 does not cure the [PSI(+)] phenotype or solubilize detectable amounts of eRF3. Third, prion-curing properties of overexpressed HSP104p, which is required for formation and maintenance of [PSI(+)], were not modified by excess Pab1p.
MH Carrier Proteins/metabolism; Fungal Proteins/genetics/*metabolism; *Gene Expression Regulation, Fungal; Human; Peptide Termination Factors/genetics/*metabolism; Poly(A)-Binding Proteins; Protein Binding; RNA-Binding Proteins/genetics/*metabolism; Receptors, Cytoplasmic and Nuclear/genetics/metabolism; Saccharomyces cerevisiae/*genetics/metabolism; Support, Non-U.S. Gov't; *Translation, Genetic; Two-Hybrid System Techniques
AD Universite de Rennes 1, CNRS UMR 6061, 35043 Rennes Cedex, France.
SP englisch
PO USA