NR ACYL
AU Crozet,C.A.; Bencsik,A.A.; Flamant,F.; Lezmi,S.; Samarut,J.; Baron,T.G.M.
TI Florid plaques in ovine PrP transgenic mice infected with an experimental ovine BSE
QU EMBO Reports 2001 Oct; 2(10): 952-6
IA http://www.nature.com/embor/journal/v2/n10/pdf/embor317.pdf
PT journal article
AB The occurrence of the variant Creutzfeldt-Jakob disease (vCJD), related to bovine spongiform encephalopathy (BSE), raises the important question of the sources of human contamination. The possibility that sheep may have been fed with BSE-contaminated foodstuff raises the serious concern that BSE may now be present in sheep without being distinguishable from scrapie. Sensitive models are urgently needed given the dramatic consequences of such a possible contamination on animal and human health. We inoculated transgenic mice expressing the ovine PrP gene with a brain homogenate from sheep experimentally infected with BSE. We found numerous typical florid plaques in their brains. Such florid plaques are a feature of vCJD in humans and experimental BSE infection in macaques. Our observation represents the first description, after a primary infection, of this hallmark in a transgenic mouse model. Moreover, these mice appear to be a promising tool in the search for BSE in sheep.
MH Animal; Blotting, Western; Brain/metabolism/pathology; Cattle; Congo Red/pharmacology; Creutzfeldt-Jakob Syndrome/transmission; Dyes/pharmacology; Encephalopathy, Bovine Spongiform/*genetics/*metabolism; Genotype; Human; Macaca; Mice; Mice, Transgenic; Prions/*biosynthesis/*genetics/metabolism; Sheep; Species Specificity; Support, Non-U.S. Gov't; Time Factors
AD Carole Crozet (ccrozet@ens-lyon.fr), Anna A. Bencsik (a.bencsik@lyon.afssa.fr), Stéphane Lezmi (s.lezmi@lyon.afssa.fr), Thierry G. M. Baron (t.baron@lyon.afssa.fr), Unité Agents Transmissibles Non Conventionnels (ATNC), Agence Francaise de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, 31 Avenue Tony Garnier, 69364 Lyon cedex 07, France; Frederic Flamant, Jacques Samarut, Laboratoire de Biologie Moléculaire et Cellulaire, Ecole Normale Supérieure de Lyon, Lyon, France
SP englisch
PO England