NR ACZW
AU Dagkessamanskaia,A.; Ter-Avanesyan,M.D.; Mager,W.H.
TI Transcriptional regulation of SUP35 and SUP45 in Saccharomyces cerevisiae
QU Yeast 1997 Oct; 13(13): 1265-74
PT journal article
AB SUP35 and SUP45 encode translational release factors in the yeast Saccharomyces cerevisiae. In addition, Sup35p is related to the cytoplasmically inherited prion-like phenotype [PSI+]. The vital cellular role of Sup35p and Sup45p prompted us to study the regulation of transcription of the corresponding genes. Since the [PSI] state of the yeast strain affects the abundance of Sup35p and Sup45p, both [PSI+] and [psi-] variants were included in these analyses. It turned out that SUP35 and SUP45 transcript levels are regulated by nutritional changes and stress in a way strikingly similar to those of ribosomal protein genes. The [PSI] state did not influence the respective transcript levels nor their regulation, although HSP12 (as a monitor of general stress-responsive) gene expression appeared to differ in the two variant strains. The transcription activation sites of SUP35 and SUP45 were mapped using deletion analysis of the respective promoter-reporter fusion genes. The UAS in both cases was found to consist of an Abf1p-site and a T-rich element. Also in this respect SUP35 and SUP45 show a notable resemblance with ribosomal protein genes. Evidence was found that SUP35 in addition harbors a potential internal promoter element which became active after progressive 5'-deletion removing the first of the three in-frame ATGs.
MH Base Sequence; Comparative Study; DNA Mutational Analysis; Fungal Proteins/biosynthesis/*genetics; *Gene Expression Regulation, Fungal; Genes, Reporter; Molecular Sequence Data; Mutagenesis; Promoter Regions (Genetics); RNA, Fungal/analysis; RNA, Messenger/analysis; Saccharomyces cerevisiae/*genetics; Sequence Deletion; Support, Non-U.S. Gov't; Suppression, Genetic; *Transcription, Genetic
AD Adilia Dagkessamanskaia (der Artikel enthält die später nicht mehr verwendete Schreibweise Adilia Dagkessamanskaya), Michael Ter-Avanesyan, Institute of Experimental Cardiology, Cardiology Research Center, Cherepkovskaya Str. 15A, 121552 Moscow, Russia; Willem H. Mager, Department of Biochemistry and Molecular Biology, IMBW, BioCentrum Amsterdam, Vrije Universiteit, de Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
SP englisch
PO England