NR ADEP
AU Dees,C.; German,T.L.; Wade,W.F.; Marsh,R.F.
TI Characterization of proteins in membrane vesicles from scrapie-infected hamster brain
QU Journal of General Virology 1985 Apr; 66(4): 851-9
PT journal article
AB Previous studies have shown that the scrapie agent is highly membrane-associated. We examined the protein composition of gradient fractions enriched for large membrane vesicles prepared from scrapie-infected and uninfected hamster brain using various methods to extract membrane proteins. We also examined proteins in detergent-extracted membrane vesicles fractionated on CsCl gradients. No qualitative differences in protein composition were seen comparing scrapie-infected and uninfected samples by one-dimensional gel electrophoresis. Extraction of proteins from membrane vesicles by phenol, pyridine, perchloric acid or lithium diiodosalicylate also failed to reveal any unique proteins in scrapie-infected hamster brain. Attempts to solubilize hydrophobic proteins (proteolipids) from CsCl gradient fractions into organic solvents were unsuccessful. These findings indicate that any hydrophobic protein associated with the scrapie agent is not a proteolipid, and that the ability of solvents to reduce scrapie infectivity is not a result of extraction of a proteolipid.
MH Animal; *Brain Chemistry; Centrifugation, Density Gradient; Glycoproteins/analysis; Hamsters; Membrane Proteins/*analysis; Molecular Weight; Prions/pathogenicity; Scrapie/*metabolism; Sheep; Support, Non-U.S. Gov't; Viral Proteins/*analysis
SP englisch
PO England