NR ADEW
AU Deininger,M.H.; Meyermann,R.; Schluesener,H.
TI Detection of two transforming growth factor-beta-related morphogens, bone morphogenetic proteins-4 and -5, in RNA of multiple sclerosis and Creutzfeldt-Jakob disease lesions
QU Acta Neuropathologica 1995 Jul; 90(1): 76-9
PT journal article, Tech. Note
AB The bone morphogenetic proteins (BMPs) constitute a novel subfamily of the transforming growth factor type beta (TGF-beta) supergene family. Here we demonstrate, using polymerase chain reaction (PCR) BMP-4 and BMP-5 messages in RNA isolated from multiple sclerosis (MS) plaque tissue. This is the first demonstration of BMP expression in an inflammatory lesion in general, and in MS in particular. However, BMP-4 and BMP-5 messages could be detected in RNA isolated from a Morbus Creutzfeldt-Jakob (CJD) lesion. Even in normal brain, RNA expression of BMP-4, but not that of BMP-5, was detected. Therefore, BMP-5 gene expression seems to be associated with MS and CJD lesions, whereas the BMP-4 gene appears to be constitutively expressed in the human brain. As TGF-beta s and BMPs are regulators of regenerative processes and contribute to regulation of chemoattraction and local immunoreactivity, BMP-4 and BMP-5 might be involved in aspects of MS lesion formation unknown so far. PCR analysis of human cell lines demonstrate BMP-4 and BMP-5 expression in leukocytic cells, suggesting that infiltrating leukocytes contribute at least in part to BMP-4 and BMP-5 mRNAs of the MS plaque.
IN Das Knochenmorphogenprotein 5 wird von Patienten mit der Creutzfeldt-Jakob-Krankheit und multipler Sklerose, aber nicht in normalen Menschen exprimiert.
MH Creutzfeldt-Jakob Syndrome/*genetics; Gene Expression; Human; Middle Age; Multiple Sclerosis/*genetics; Polymerase Chain Reaction; Proteins/*analysis; RNA/*analysis; Support, Non-U.S. Gov't; Transforming Growth Factor beta/*analysis
AD Institute of Brain Research, University of Tübingen, Calwer Str 3, D-72076 Tübingen, Germany.
SP englisch
PO Deutschland