NR ADGI
AU Denman,R.B.; Purow,B.; Rubenstein,R.; Miller,D.L.
TI Hammerhead ribozyme cleavage of hamster prion pre-mRNA in complex cell-free model systems
QU Biochemical and Biophysical Research Communications 1992 Jul 31; 186(2): 1171-7
PT journal article
AB The cleavage properties of a trans-acting hammerhead ribozyme targeted 51 bases upstream of the putative splicing branch point in the hamster prion pre-mRNA intron were investigated in cell-free model systems in vitro. The specificity of cleavage was demonstrated by the inability of this ribozyme to cleave a non-homologous synthetic message encoding part of the beta amyloid peptide precursor, beta APP, and by the inability of the prion pre-mRNA to be cleaved by a ribozyme targeted to beta amyloid peptide precursor mRNA. Also, the addition of total RNA isolated from rat brain had only a minimal effect on the cleavage of the prion substrate pre-mRNA by the ribozyme. Finally neither the presence of 100 ng of nuclear or cytoplasmic proteins were found to affect the rate of cleavage in vitro.
MH Adenosine Triphosphate/metabolism; Amyloid beta-Protein Precursor/genetics; Animal; Base Sequence; Brain/physiology; Cell-Free System; Cloning, Molecular; Hamsters; Molecular Sequence Data; Nucleic Acid Conformation; Prions/*genetics; RNA/genetics; RNA Precursors/genetics/*metabolism; RNA, Catalytic/genetics/*metabolism; RNA, Messenger/*genetics; Rats; Restriction Mapping; Sharks; Substrate Specificity; *Transcription, Genetic
AD New York State Institute for Basic Research in Developmental Disabilities, Staten Island 10314.
SP englisch
PO USA