NR ADGJ
AU Denning,C.; Burl,S.; Ainslie,A.; Bracken,J.; Dinnyes,A.; Fletcher,J.; King,T.; Ritchie,M.; Ritchie,W.A.; Rollo,M.; de Sousa,P.; Travers,A.; Wilmut,I.; Clark,A.J.
TI Deletion of the alpha(1,3)galactosyl transferase (GGTA1) gene and the prion protein (PrP) gene in sheep
QU Nature Biotechnology 2001 Jun; 19(6): 559-62
KI Nat Biotechnol. 2001 Jun;19(6):529-30. PMID: 11385449
PT journal article
AB Nuclear transfer offers a cell-based route for producing precise genetic modifications in a range of animal species. Using sheep, we report reproducible targeted gene deletion at two independent loci in fetal fibro-blasts. Vital regions were deleted from the alpha(1,3)galactosyl transferase (GGTA1) gene, which may account for the hyperacute rejection of xenografted organs, and from the prion protein (PrP) gene, which is directly associated with spongiform encephalopathies in humans and animals. Reconstructed embryos were prepared using cultures of targeted or nontargeted donor cells. Eight pregnancies were maintained to term and four PrP-/+ lambs were born. Although three of these perished soon after birth, one survived for 12 days. These data show that lambs carrying targeted gene deletions can be generated by nuclear transfer.
MH Animal; *Animals, Genetically Modified; Animals, Newborn; Blotting, Southern; Cell Nucleus/metabolism; Exons; Fibroblasts/metabolism; Galactosyltransferases/*genetics; *Gene Deletion; Gene Targeting; *Gene Transfer Techniques; Models, Genetic; Prions/*genetics; Sheep; Support, Non-U.S. Gov't; Time Factors; Transfection
AD Department of Gene Expression and Development, Roslin Institute, Roslin, Midlothian EH25 9PS, United Kingdom.
SP englisch
PO USA