NR ADIE
AU Di Martino,A.; Bigon,E.; Corona,G.; Callegaro,L.
TI Production and characterization of antibodies to mouse scrapie-amyloid protein elicited by non-carrier linked synthetic peptide immunogens
QU Journal of Molecular Recognition 1991 Mar-Jun; 4(2-3): 85-91
PT journal article
AB Two polyclonal antibodies were raised by immunizing rabbits with two non carrier-linked synthetic peptides whose amino acid sequences corresponded to codons 89-107 (peptide P1) and 219-233 (peptide P2) of the translated cDNA sequence of murine PrP protein. These free peptides, whose structural characteristics in solution were studied by circular dichroism, elicited a reasonable immunologic response in animals. Both antibodies still recognized the corresponding immunogens after affinity chromatography purification. However, only antibodies raised to the former sequence reacted by immunoblot with a purified preparation of murine scrapie amyloid protein. These findings are discussed together with their correlation to peptide structure and the effectiveness of this simplified immunization procedure.
MH Amino Acid Sequence; Animal; Antibodies, Viral/*immunology/isolation & purification; Antigens, Viral/immunology; Chromatography, High Pressure Liquid; Circular Dichroism; Immunization; Male; Mice; Molecular Sequence Data; Peptides/chemical synthesis/immunology; PrPsc Proteins; Prions/*immunology; Rabbits
AD Advanced Technology Division, Fidia SpA, Abano Terme, Italy.
SP englisch
PO England
OR Prion-Krankheiten D