NR ADKG
AU Diez,M.; Koistinaho,J.; DeArmond,S.J.; Camerino,A.P.; Groth,D.; Cayetano-Canlas,J.; Prusiner,S.B.; Hokfelt,T.
TI Aberrant induction of neuropeptide Y mRNA in hippocampal CA3 pyramidal neurones in scrapie-infected mice
QU Neuroreport 1996 Aug 12; 7(12): 1887-92
PT journal article
AB The neurochemical alterations preceding neurological dysfunction and neuronal death in prion diseases are not well characterized. Here we examined, using in situ hybridization histochemistry, the expression of neuropeptide Y (NPY), an inducible and abundant neuropeptide in mammalian brain with known neuroregulatory functions, and glial fibrillary acidic protein (GFAP), a marker for astroglial activation, in the hippocampus at different time points following intracerebral prion inoculation in male CD-1 mice. Between 110 and 140 days postinoculation NPY mRNA expression was specifically up-regulated in CA3 pyramidal neurones, whereas expression of NPY in hilar neurones remained unaltered. Up-regulation of GFAP mRNA was observed in the CA1 stratum radiatum at 60 days, and spread throughout the hippocampus, cortex and thalamus between 110 and 140 days, suggesting early accumulation of scrapie prion protein in these regions. The clinical symptoms were first manifested 120 days postinoculation. Aberrant induction of NPY mRNA in the hippocampal CA3 pyramidal neurones preceded the onset of neurological symptoms, and may be involved in the regulation of glutamate release at the Schaffer collateral-CA1 synapses in scrapie-infected mice.
IN Scrapie-infizierte männliche CD-1-Mäuse zeigten frühestens 120 Tage nach einer intrazerebralen Inokulation klinische Symptome. Bereits 60 Tage nach der Inokulation wurde eine Zunahme der mRNA für das glial fibrillary acidic protein GFAP im stratum radiatum festgestellt. Zwischen dem 110. und dem 140. Tag wurde diese verstärkte Expression im ganzen Hippokampus, sowie in Cortex und Thalamus beobachtet. Die mRNA für das Neuropeptid Y war zwischen dem 110. und dem 140. Tag in CA3 pyramidalen Neuronen des Hippokampus vermehrt, in Hilus-Neuronen hingegen unverändert.
MH Animal; Autoradiography; Hippocampus/*metabolism; In Situ Hybridization; Male; Mice; Mice, Inbred Strains; Neuropeptide Y/*metabolism; Pyramidal Cells/*metabolism; RNA, Messenger/metabolism; Scrapie/*metabolism; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.
AD Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
SP englisch
PO England