NR ADMO
AU Doh-ura,K.; Perryman,S.; Race,R.E.; Chesebro,B.
TI Identification of differentially expressed genes in scrapie-infected mouse neuroblastoma cells
QU Microbial Pathogenesis 1995 Jan; 18(1): 1-9
PT journal article
AB In vitro cDNA libraries from scrapie-infected and non-infected murine neuroblastoma cell lines were screened with cDNA probes derived by subtractive hybridization from scrapie-infected and uninfected cells to identify genes with altered expression associated with scrapie infection. Eleven independent recombinant clones, whose expression was either increased or decreased in scrapie-infected cells, were identified. Expression of these genes was also analyzed in a panel of scrapie-infected and non-infected cell lines. Five genes had altered mRNA expression in most scrapie-infected neuroblastoma cell clones compared to non-infected clones. These genes were chromogranin B, intracisternal-A particle envelope, ornithine decarboxylase antizyme, heat shock protein 70 and one unidentified gene. None of these alterations in gene expression was uniquely scrapie-specific; however, the skewed association of increased expression with scrapie infection suggested that a causal relationship might exist between scrapie infection and altered expression of these genes in mouse neuroblastoma cells in vitro.
IN In scrapieinfizierten Mausneuroblastomzellen wird eine ganze Reihe von Genen angeschaltet, die wegen fehlender Spezifität aber nicht als Marker taugen.
ZR 29
MH Animal; Blotting, Northern; Brain/metabolism; Clone Cells; DNA, Complementary/genetics; *Gene Expression Regulation; Gene Library; Mice; Nervous System/*metabolism; Neuroblastoma; Nucleic Acid Hybridization; RNA Processing, Post-Transcriptional; RNA, Messenger/*analysis; Scrapie/*genetics; Selection (Genetics); Sequence Analysis, DNA; Support, Non-U.S. Gov't; Tumor Cells, Cultured
AD Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840, USA
SP englisch
PO England