NR ADQO
AU Duittoz,A.H.; Hevor,T.
TI Primary culture of neural precursors from the ovine central nervous system (CNS)
QU Journal of Neuroscience Methods 2001 May 30; 107(1-2): 131-40
PT journal article
AB The present study demonstrates that bipotential neural precursors isolated from an early developmental stage of the sheep embryo nervous system can be maintained in vitro in an undifferentiated state for a long period. These precursors multiplied under the action of epidermal growth factor and basic fibroblast growth factor and formed free-floating aggregates of nestin-immunoreactive cells, called neurospheres. These precursors can undergo predominantly neural or glial differentiation according to the culture conditions. Medium supplemented with foetal calf serum mainly favoured cell differentiation predominantly into astrocytes, whereas the defined SATO medium favoured neuronal differentiation. Using various immunomarkers of neurones and astroglial cells, we described the course of differentiation of neuronal and astroglial cells in different culture conditions. The ability to grow neural precursors from common laboratory animals has been useful for studying the cellular and molecular mechanisms underlying the development of the central nervous system. Furthermore, neural progenitors are already being used for in vivo cell therapy in various neurodegenerative disorders. The ovine species is a well-known model for prion diseases, since scrapie is endemic in most countries and has been studied for a long time. In this respect, the availability of ovine neural precursors will add a new perspective to the study of the pathogenicity of prion diseases.
MH Animal; Blood Proteins/pharmacology; Brain Tissue Transplantation/methods; Cell Culture/*methods; Cell Differentiation/drug effects/physiology; Cell Lineage/drug effects/physiology; Cells, Cultured/cytology/drug effects/*metabolism; Central Nervous System/*cytology/*embryology/metabolism; Culture Media/metabolism; Epidermal Growth Factor/pharmacology; Female; Fetus; Glial Fibrillary Acidic Protein/metabolism; Immunohistochemistry; Kinetics; Microtubule-Associated Proteins/metabolism; Nerve Growth Factors/pharmacology; Neuroglia/cytology/metabolism; Neurons/*cytology/drug effects/metabolism; Scrapie/pathology/physiopathology; Sheep/anatomy & histology/*embryology/metabolism; Stem Cells/*cytology/drug effects/metabolism; Tubulin/metabolism
AD UMR 6073 INRA Universite de Tours, PRC Neuroendocrinologie Sexuelle, INRA, F-37380, Nouzilly, France. duittoz@tours.inra.fr
SP englisch
PO Niederlande