NR ADST
AU Eikelenboom,P.; Scott,J.R.; McBride,P.A.; Rozemuller,J.M.; Bruce,M.E.; Fraser,H.
TI No evidence for involvement of plasma proteins or blood-borne cells in amyloid plaque formation in scrapie-affected mice. An immunohistoperoxidase study.
QU Virchows Archiv. B, Cell Pathology Including Molecular Pathology 1987; 53(4): 251-6
PT journal article
AB The present study was designed to investigate blood-brain permeability and the possible involvement of plasma proteins and blood-borne cells in amyloid plaque formation in scrapie-affected mice. No abnormal extravasation of intravenously injected horseradish peroxidase (HRP) was found and with immunocytochemical techniques no plasma proteins were detected in neuropil from scrapie-affected mice. In contrast to an earlier report, these findings suggest that the blood-brain barrier is essentially intact in scrapie-affected mice. Using immunohistochemical and enzyme histochemical methods no cells belonging to the monocyte-macrophage lineage were detected in association with amyloid plaques. Thus, by these methods there was no evidence that plasma proteins or blood-borne cells are involved in amyloid plaque formation in scrapie-affected mice. However, astrocytes were consistently found to be associated with amyloid plaques at all stages of their development.
MH Amyloid/*biosynthesis; Animal; Blood-Brain Barrier; Brain/enzymology; Horseradish Peroxidase/metabolism; Immunoenzyme Techniques; Immunohistochemistry; Mice; Mice, Inbred Strains; Scrapie; Support, Non-U.S. Gov't
AD P. Eikelenboom, Department of Psychiatry, Medical Faculty, Free University, Amsterdam, The Netherlands; Patricia A. McBride (tricia.mcbride@bbsrc.ac.uk), Moira E. Bruce (moira.bruce@bbsrc.ac.uk), Hugh Fraser (HughFraser@bbsrc.ac.uk), Institute for Animal Health, BBSRC and MRC, Neuropathogenesis Unit, Ogston Building, West Mains Road, Edinburgh EH9 3JF, Scotland, UK
SP englisch
PO Deutschland