NR ADUH
AU Ernst,D.R.; Race,R.E.
TI Comparative analysis of scrapie agent inactivation methods
QU Journal of Virological Methods 1993 Feb; 41(2): 193-201
IA http://www.cwd.cc/Comparative%20Analysis%20of%20Scrapie%20Agent%20Inactivation%20Methods.pdf
PT journal article
AB A scrapie-infected hamster brain homogenate was subjected to several different potential inactivation methods. Methods included autoclaving for various lengths of time, either alone or in combination with different concentrations of sodium hydroxide or LpH, an aqueous acid phenolic derivative (Calgon Vestal Laboratories in St. Louis, MO). Inactivation treatments utilizing either NaOH or LpH alone were also evaluated. It was determined that several of the treatments inactivated all of the detectable infectivity.
IN Mit einigen für den Hausgebrauch kaum praktikablen Methoden (Autoklavieren) und Chemikalien (Natronlauge) läßt sich das infektiöse Potential scrapieverseuchter Hamstergehirnhomogenate angeblich vollständig abbauen. Beide Wege wurden durch andere Publikationen widerlegt.
MH Animal; Brain/microbiology; Comparative Study; Disinfection/*methods; Hamsters; Heat; Phenols/pharmacology; *Prions/drug effects/pathogenicity; Sodium Hydroxide/pharmacology
AD National Institutes of Health, National Institute of Allergy and Infectious Diseases, Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, Hamilton, MT 59840.
SP englisch
PO Niederlande