NR ADVC
AU Esposito,F.; Ammendola,R.; Duilio,A.; Costanzo,F.; Giordano,M.; Zambrano,N.; D'Agostino,P.; Russo,T.; Cimino,F.
TI Isolation of cDNA fragments hybridizing to rat brain-specific mRNAs
QU Developmental Neuroscience 1990; 12(6): 373-81
PT journal article
AB A cDNA minilibrary in pUC18 has been generated from 3-month-old rat brains. Two hundred clones were randomly selected and sequenced. Comparison with a nucleic acid and protein data bank revealed a number of cDNA fragments not homologous to any published sequence. Northern blot analyses of some of these clones yielded 5 brain-specific cDNA fragments. The full-length cDNA for one of these clones has been isolated and completely sequenced. It corresponds to an mRNA of about 1,500 nucleotides, which is present in brain and, to a lesser extent, in heart and skeletal muscle. Its expression is developmentally regulated in the rat brain from 14-day embryos to 3-month-old adults. A very recent comparison with the EMBL nucleotide sequence data bank showed that this mRNA codes for a brain-specific snRNP-associated protein.
MH Aging; Amino Acid Sequence; Animal; Base Sequence; Blotting, Southern; Brain/embryology/growth & development/*physiology; Comparative Study; DNA/*genetics/isolation & purification; Gene Library; Gestational Age; Mice; Molecular Sequence Data; Nerve Tissue Proteins/*genetics; Nucleic Acid Hybridization; Organ Specificity; Plasmids; PrPsc Proteins; Prions/genetics; RNA, Messenger/*genetics; Rats; Rats, Inbred Strains; Sequence Homology, Nucleic Acid; Support, Non-U.S. Gov't; Viral Proteins/genetics
AD Dipartimento di Biochimica e Biotecnologie Mediche, Universita di Napoli, Italia.
SP englisch
PO Schweiz