NR ADWK
AU Farquhar,C.F.; Dornan,J.; Somerville,R.A.; Tunstall,A.M.; Hope,J.
TI Effect of Sinc genotype, agent isolate and route of infection on the accumulation of protease-resistant PrP in non-central nervous system tissues during the development of murine scrapie
QU Journal of General Virology 1994 Mar; 75(3): 495-504
PT journal article
AB Mice congenic for the Sinc gene were infected intracerebrally with two scrapie strains, ME7 and 22A. At various times during the incubation period tissues were monitored for the infection-specific form of PrP (PrPsc). PrPsc was found in brain, spleen, lymph nodes, pancreas, submaxillary gland and thymus. After intraperitoneal inoculation PrPsc was found in spleen, lymph nodes, pancreas and submaxillary glands prior to its detection in brain. The kinetics of accumulation of PrPsc in these tissues was dependent on the infecting strain of agent, on the mouse Sinc genotype and on the route of infection. This study supports using the presence of PrPsc as an indicator of infectivity in brain and extraneural tissues and defines some of the parameters which influence when and where PrPsc is first found.
IN In mit zwei Scrapiestämmen intracerebral infizierten Mäusen wurden die veränderten Prionproteine in Gehirn, Milz, Lymphknoten, Pankreas, Speicheldrüse und Thymus gefunden. Nach Injektionen unters Bauchfell wurden die veränderten Prionproteine in Milz, Lymphknoten, Pankreas und Speicheldrüse gefunden, bevor sie auch im Gehirn auftauchten. Wo die veränderten Prionproteine wann auftauchen, das hängt vom Infektionsort, dem Scrapiestamm und dem Sine-Genotyp der Empfängermaus ab.
MH Animal; Brain/microbiology; Disease Models, Animal; Endopeptidase K; Genotype; Kinetics; Lymphoid Tissue/*microbiology; Mice; Mice, Inbred Strains; Pancreas/*microbiology; PrPsc Proteins; Prions/drug effects/genetics/*isolation & purification/*metabolism; Scrapie/*microbiology; Serine Endopeptidases
AD Institute for Animal Health, AFRC and MRC Neuropathogenesis Unit, Edinburgh, U.K.
SP englisch
PO England