NR AEAW
AU Fischer,M.; Rülicke,T.; Raeber,A.J.; Sailer,A.; Moser,M.; Oesch,B.; Brandner,S.; Aguzzi,A.; Weissmann,C.
TI Prion protein (PrP) with amino-proximal deletions restoring susceptibility of PrP knockout mice to scrapie
QU EMBO Journal 1996 Mar 15; 15(6): 1255-64
PT journal article
AB The 'protein only' hypothesis postulates that the prion, the agent causing transmissible spongiform encephalopathies, is PrPsc, an isoform of the host protein PrPc. Protease treatment of prion preparations cleaves off approximately 60 N-terminal residues of PrPsc but does not abrogate infectivity. Disruption of the PrP gene in the mouse abolishes susceptibility to scrapie and prion replication. We have introduced into PrP knockout mice transgenes encoding wild-type PrP or PrP lacking 26 or 49 amino-proximal amino acids which are protease susceptible in PrPsc. Inoculation with prions led to fatal disease, prion propagation and accumulation of PrPsc in mice expressing both wild-type and truncated PrPs. Within the framework of the 'protein only' hypothesis, this means that the amino-proximal segment of PrPc is not required either for its susceptibility to conversion into the pathogenic, infectious form of PrP or for the generation of PrPsc.
ZR 55
MH Amino Acid Sequence; Amyloid/deficiency/genetics; Animal; Base Sequence; Brain/anatomy & histology; Comparative Study; Disease Susceptibility; Mice; Mice, Knockout; Molecular Sequence Data; Mutation; Nerve Tissue Proteins/biosynthesis/*genetics; PrPc Proteins/genetics; PrPsc Proteins/biosynthesis/genetics; Prions/biosynthesis/*genetics; Protein Precursors/deficiency/genetics; RNA, Messenger/isolation & purification; Scrapie/etiology/*genetics/transmission; Sequence Deletion; Support, Non-U.S. Gov't
AD Marek Fischer, Alex Raeber und Prof. Charles Weissmann, Universität Zürich, Institut für Molekularbiologie, Abteilung I, Hönggerberg, 8093 Zürich, Schweiz, Postfach, Tel. 633 24 91, Fax 371 72 05; ; Thomas Rülicke, Biologisches Zentrallabor im Universitätsspital Zürich; Sebastian Brandner und Adriano Aguzzi, Institut für Neuropathologie im Universitätsspital Zürich; Bruno Oesch, Institut für Hirnforschung der Universität Zürich; Andreas Sailer, jetzt Salk Institut, Molecular Neurobiology Laboratory, La Jolla, Ca 92037, USA
SP englisch
PO England
OR Prion-Krankheiten 3