NR AECQ
AU Foster,J.D.; Goldmann,W.; Parnham,D.; Chong,A.; Hunter,N.
TI Partial dissociation of PrPsc deposition and vacuolation in the brains of scrapie and BSE experimentally affected goats
QU Journal of General Virology 2001 Jan; 82(1): 267-73
PT journal article
AB The diagnosis of transmissible spongiform encephalopathies (TSEs) depends on the detection of vacuolation in brain sections taken from affected individuals and/or the identification of the disease-associated isoform of the PrP (prion) protein (PrPsc). During the course of an investigation, goats clinically affected following experimental infection with three different sources of TSE (SSBP/1, CH1641 and BSE) developed widespread vacuolar degeneration in the brain. With BSE, PrPsc was clearly recognized in affected goat brain by immunocytochemistry (icc) and Western blotting, but in contrast the experimental scrapie sources SSBP/1 and CH1641 showed almost no or very little PrPsc by icc. Western blot analysis of PrPsc from BSE-affected and SSBP/1-affected goat brain showed that the protein was present in brain affected by both TSE sources, but could not be used to determine how much protein was present. It became clear that PrPsc and vacuolation could be partially dissociated following challenge with two of the three TSE sources. Subtle differences in glycosylation patterns between BSE- and SSBP/1-associated PrP protein isoforms could also be recognized, although these experimentally generated results should not be regarded as a BSE/scrapie differential test. However, our study warns that the reliance on PrPsc determination by icc alone as a means by which to diagnose TSE infection may generate false negative results.
MH Animal; Blotting, Western; Brain/*pathology/ultrastructure; Brain Chemistry; Cattle; Comparative Study; Encephalopathy, Bovine Spongiform/*pathology; False Negative Reactions; Goats; Immunohistochemistry; PrPsc Proteins/*analysis/pathogenicity; Scrapie/*pathology; Sheep
AD BBSRC Institute for Animal Health, Neuropathogenesis Unit, Kings Buildings, West Mains Road, Edinburgh EH9 3JF, UK. Jim.Foster@BBSRC.ac.uk
SP englisch
PO England
EA pdf-Datei und HTML-Version
OR Prion-Krankheiten 3