NR AEGY
AU Gabizon,R.; Telling,G.; Meiner,Z.; Halimi,M.; Kahana,I.; Prusiner,S.B.
TI Insoluble wild-type and protease-resistant mutant prion protein in brains of patients with inherited prion disease
QU Nature Medicine 1996 Jan; 2(1): 59-64
PT journal article
AB We studied prion proteins (PrP) in skin and brains of Libyan Jews carrying the E200K mutation who died of familial Creutzfeldt-Jakob disease (CJD). Unexpectedly, studies with brain showed that PrP molecules encoded both by the wild-type (wt) and mutant alleles exhibit altered properties characteristic of the prion protein associated with prion diseases (PrPsc). Using monospecific antisera, we found that wtPrP was insoluble in the brains of three patients who were heterozygous for the E200K mutation, whereas mutant PrP was both insoluble and protease-resistant. Our results argue that both wild-type and mutant PrP undergo conformational changes and are particularly intriguing, because the normal isoform PrPc is soluble in nondenaturing detergents and is readily digested by proteases, whereas PrPsc is insoluble and resistant to proteolytic digestion. Our findings indicate that insoluble wtPrP represents a conformational intermediate, the first to be identified, within a pathway in which PrPc is converted to PrPsc.
ZR 47
MH Amino Acid Sequence; Animal; Antibodies, Monoclonal; Brain/metabolism; Cells, Cultured; Creutzfeldt-Jakob Syndrome/genetics/metabolism; Endopeptidases; Fibroblasts/metabolism; Heterozygote Detection; Homozygote; Human; Israel; Jews; Libya/ethnology; Mice; Mice, Transgenic; Molecular Sequence Data; Peptides/chemistry/immunology; *Point Mutation; Prions/chemistry/*genetics/metabolism; Skin/metabolism; Solubility; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.
AD Department of Neurology, Hadassah University Hospital, Ein Karem, Jerusalem, Israel.
SP englisch
PO USA
OR Prion-Krankheiten G