NR AEHJ
AU Gabriel,J.M.; Oesch,B.; Kretzschmar,H.A.; Scott,M.R.D.; Prusiner,S.B.
TI Molecular cloning of a candidate chicken prion protein
QU Proceedings of the National Academy of Sciences of the United States of America 1992 Oct 1; 89(19): 9097-101
PT journal article
AB Fractions enriched for acetylcholine receptor-inducing activity from chicken brain were found to contain a protein that was approximately 30% homologous with mammalian prion proteins [Harris, D. A., Falls, D. L., Johnson, F. A. & Fischbach, G. D. (1991) Proc. Natl. Acad. Sci. USA 88, 7664-7668]. To extend these observations, we recovered genomic clones encoding a putative chicken prion protein (PrP). Like mammalian PrP molecules, the candidate chicken PrP is encoded by a single-copy gene and the entire open reading frame is found within a single exon. All of the structural features of mammalian PrP were found in the chicken protein. When the N-terminal repeats of PrP were not considered, the chicken and mammalian proteins were approximately 55% homologous, allowing for conservative substitutions. Screening of a chicken genomic DNA library failed to identify a more closely related chicken PrP homologue. These findings argue that the protein which purifies with acetylcholine receptor-inducing activity is chicken PrP.
IN Auch bei Hühnern gibt es ein Prionprotein, welches ebenfalls von nur einem Exon kodiert wird, in nur einer Kopie vorliegt und strukturell den Säugerprionproteinen recht ähnlich zu sein scheint. Dieses Prionprotein scheint ein Aktivator des Acetylcholinrezeptors zu sein.
MH Amino Acid Sequence; Animal; Base Sequence; Blotting, Southern; Chickens; Cloning, Molecular/methods; Comparative Study; DNA/genetics/isolation & purification; Genomic Library; Human; Molecular Sequence Data; Oligonucleotide Probes; Open Reading Frames; Phylogeny; Prions/*genetics; Sequence Homology, Amino Acid; Support, U.S. Gov't, Non-P.H.S.; Support, U.S. Gov't, P.H.S.
AD Department of Neurology, University of California, San Francisco 94143.
SP englisch
PO USA
OR Prion-Krankheiten G