NR AEQE
AU Glover,K.J.; Martini,P.M.; Vold,R.R.; Komives,E.A.
TI Preparation of insoluble transmembrane peptides: glycophorin-A, prion (110-137), and FGFR (368-397).
QU Analytical Biochemistry 1999 Aug 1; 272(2): 270-4
PT journal article
AB A general procedure for the reliable preparation of large quantities of insoluble transmembrane peptides has been developed. Optimal couplings were obtained during synthesis by using high-temperature couplings in conjunction with O-(7-azabenzotriazol-1-yl)-1,1,3, 3-tetramethyluronium hexafluorophosphate (HATU) activation. Improved purification schemes were developed that use reverse- phase HPLC on a C1 column and elution with a 2:1 mixture of 1-propanol:1-butanol. Using these methods three very different transmembrane peptides all longer than 25 amino acids have been prepared: glycophorin-A, prion (110-137), and fibroblast growth factor receptor (368-397).
MH Amino Acid Sequence; Chromatography, High Pressure Liquid/methods; Glycophorin/*chemical synthesis/chemistry; Indicators and Reagents; Molecular Sequence Data; Peptide Fragments/*chemical synthesis/chemistry; Prions/*chemical synthesis/chemistry/isolation & purification; Receptors, Fibroblast Growth Factor/*chemistry; Spectrum Analysis, Mass/methods; Support, U.S. Gov't, P.H.S.
AD Kerney Jebrell Glover, Paul M. Martini, Regitze R. Vold and Elizabeth A. Komives (ekomives@ucsd.edu), Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0601, USA
SP englisch
PO USA