NR AEQI
AU Godec,M.S.; Asher,D.M.; Kozachuk,W.E.; Masters,C.L.; Rubi,J.U.; Payne,J.A.; Rubi-Villa,D.J.; Wagner,E.E.; Rapoport,S.I.; Schapiro,M.B.
TI Blood buffy coat from Alzheimer's disease patients and their relatives does not transmit spongiform encephalopathy to hamsters
QU Neurology 1994 Jun; 44(6): 1111-5
PT journal article
AB There was a report of spongiform encephalopathy transmitted to Syrian hamsters by intracerebral inoculation with the blood buffy coat of patients with Alzheimer's disease (AD) and their unaffected first-degree relatives. We attempted to verify that report, taking measures to reduce the risk of contaminating samples with agents causing spongiform encephalopathies. We obtained blood from 50 subjects, including six patients with familial AD, 21 unaffected first-degree relatives (siblings and offspring) of patients with familial AD, and 20 control subjects. We inoculated the buffy coats intracerebrally into Syrian LVG hamsters, observed them for signs of neurologic disease, examined their brains for neuropathologic changes at time of death, and performed serial (blind) passages by inoculating suspensions of all recovered brains into fresh LVG hamsters. We discerned no clinical illness or histopathologic changes resembling experimental spongiform encephalopathy in any hamster inoculated with human buffy coat nor in blind-passage hamsters, nor were the life spans of those hamsters shortened. We conclude that AD is not caused by an agent that transmits spongiform encephalopathy to hamsters.
IN Die Autoren behaupten erfolglos versucht zu haben, die von Manuelidis berichteten erfolgreichen Übertragungen von CJK durch Blut gesunder Menschen auf Nager zu wiederholen. In Wirklichkeit haben sie aber nur erfolglos versucht, Alzheimer auf syrische Hamster zu übertragen.
MH Alzheimer Disease/blood/*complications/*genetics; Animal; Female; Hamsters; Human; Leukocyte Transfusion; *Leukocytes; Male; Mesocricetus; Prion Diseases/*etiology; Support, Non-U.S. Gov't
AD Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, Bethesda, MD 20892.
SP englisch
PO USA