NR AESR
AU Gonzalez,L.; Martin,S.; Begara-McGorum,I.M.; Hunter,N.; Houston,E.F.; Simmons,M.M.; Jeffrey,M.J.
TI Effects of agent strain and host genotype on PrP accumulation in the brain of sheep naturally and experimentally affected with scrapie
QU Journal of Comparative Pathology 2002 Jan; 126(1): 17-29
PT journal article
AB Different cellular and neuroanatomical types of disease-specific prion protein (PrP(d)) accumulation in the brain were identified in sheep of different breeds and PrP genotypes exposed to experimental or natural scrapie infection. Immunohistochemical examination of the brains of 43 sheep with clinical signs compatible with scrapie revealed 12 different PrP(d)types, which were subjectively quantified in eight different brain regions. The PrP(d)types were grouped into four PrP(d)patterns, the relative magnitude of which provided the PrP(d)profile of each sheep examined. The analysis of the differences in magnitude and relative proportion of each of these PrP(d)types and patterns indicated (1) an effect of the scrapie strain on the PrP(d)profile, and (2) a possible effect of the host genotype on the magnitude of PrP(d)accumulation in the brain, apparently related to the incubation period. Furthermore, intraneuronal deposition of PrP(d)was the type most closely associated with the development of clinical disease. We conclude that different scrapie strains can be distinguished by PrP immunohistochemical examination of brains of affected animals.
MH Animal; Brain/*metabolism/pathology; Comparative Study; Genetic Predisposition to Disease; Genotype; Immunoenzyme Techniques/veterinary; Neuroglia/metabolism/pathology; Neurons/metabolism/pathology; PrPsc Proteins/classification/*genetics/metabolism; Scrapie/*genetics/metabolism/pathology; Sheep; Species Specificity; Support, Non-U.S. Gov't
AD Lorenzo González, Stuart Martin, Martin J. Jeffrey (m.jeffrey@vla.maff.gov.uk), VLA Lasswade Veterinary Laboratory, Pentlands Science Park, Bush Loan, Penicuik, Edinburgh EH26 OPZ, Scotland, UK; Isabel M. Begara-McGorum, Scottish Agricultural College, Veterinary Science Division, Bush Estate, Boon Loan, Penicuik, Midlothian EH26 0QE, UK; Nora Hunter (nora.hunter@bbsrc.ac.uk), Institute for Animal Health, BBSRC and MRC, Neuropathogenesis Unit, Ogston Building, West Mains Road, Edinburgh EH9 3JF, Scotland, UK; E. Fiona Houston (fiona.houston@bbsrc.ac.uk), Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berkshire RG20 7NN, UK; Marion M. Simmons, Neuropathology Unit, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone, Surrey, KT15 3NB, UK
SP englisch
PO England
OR Prion-Krankheiten G