NR AEUF
AU Graber,H.U.; Meyer,R.K.; Fatzer,R.; Vandevelde,M.; Zurbriggen,A.
TI In situ hybridization and immunohistochemistry for prion protein (PrP) in bovine spongiform encephalopathy (BSE)
QU Zentralblatt für Veterinärmedizin, Reihe A 1995 Sep; 42(7): 453-9
PT journal article
AB In about 5% of the cows showing clinical signs of bovine spongiform encephalopathy (BSE) the histopathological examination is not conclusive. In order to rule out BSE in these cases, additional methods are necessary. For that reason, non-radioactive in situ hybridization (ISH) was performed using a riboprobe against the messenger RNA coding for the prion protein (PrP). In addition, a polyclonal antibody for immunohistochemistry (IHC) was generated against a synthetic peptide derived from bovine PrP. ISH and IHC were used to analyse brain sections of cattle suffering from BSE and various neurological diseases including four cows with clinically suspect but histologically unconfirmed BSE. ISH revealed no differences in localization, distribution and neuronal levels of PrP mRNA between BSE positive and negative cattle. However, there was a BSE-specific staining pattern in IHC allowing to exclude BSE the four suspected cases. Additionally, IHC for PrP is an elegant alternative to search for scrapie associated fibrils by electron microscopy.
IN Die Autoren weisen daraufhin, dass sich Antikörper gegen Prionpeptide im Gegensatz zu Nukleinsäureproben für die Diagnose von BSE eignen und eine elegantere Alternative zu elektronenmikroskopischen Untersuchungen darstellen.
ZR 22
MH Animal; Brain/pathology/ultrastructure; *Brain Chemistry; Cattle; Encephalopathy, Bovine Spongiform/*metabolism/pathology; Female; Immunohistochemistry; In Situ Hybridization/veterinary; PrP 27-30 Protein/analysis/ultrastructure; Prions/*analysis/genetics; RNA, Messenger/analysis/genetics; Support, Non-U.S. Gov't
AD Institute of Animal Neurology, University of Bern, Switzerland.
SP englisch
PO Deutschland