NR AFAB
AU Haig,D.A.; Clarke,M.C.; Millson,G.C.
TI Failure of fluorocarbon treatment to affect sensitivity of scrapie agent to ultraviolet light
QU Journal of Comparative Pathology 1977 Jan; 87(1): 103-7
PT journal article
AB
Treatment of a suspension of brain, from scrapie affected mice, with fluorocarbon reduced the titre of the agent by about 60 per cent. A second exposure further reduced the titre by a total of 90 per cent. Dry weight and protein content of suspensions were reduced by similar amounts following fluorocarbon treatment so that no overall purification of agent was obtained. However, fluorocarbon had a more marked effect on dry weight and protein content of a fraction sedimented at 100 000 to 130 000 g.
No difference was observed in the sensitivity of scrapie activity to inactivation by u.v. light at a wavelength of 254 nm following exposure of a suspension of scrapie agent to one or two treatments with fluorocarbon.
MH Animal; Fluorocarbons/*pharmacology; Mice; Prions/analysis/drug effects/*radiation effects; *Ultraviolet Rays
AD D. A. Haig, M. C. Clarke, G. C. Millson and Tikvah Alper (Present address: Gray Laboratory, Mount Vernon Hospital, Northwood, Middlesex HA6 2RN), Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire RG16 0NN, England; Medical Research Council, Experimental Radiopathology Unit, Hammersmith Hospital, Ducane Road, London W12 0HS, England
SP englisch
PO England