NR AFFB
AU Hashimoto,K.; Mannen,T.; Nukina,N.
TI Immunohistochemical study of kuru plaques using antibodies against synthetic prion protein peptides
QU Acta Neuropathologica 1992; 83(6): 613-7
PT journal article
AB Prion protein (PrP) is a protein closely associated with the transmission of scrapie and Creutzfeldt-Jakob disease (CJD). Kuru plaques are composed of this protein. PrP33-35 is converted to protease-resistant PrP27-30 by proteinase K digestion. It has not yet been determined which of these PrPs is present in kuru plaques in vivo. Accordingly we synthesized two peptides (peptide-N and peptide-M) that, respectively, corresponded to the protease-sensitive and protease-resistant portions of PrP33-35, based on the amino acid sequence deduced from human PrP cDNA. These two synthetic peptides were used to immunize rabbits and produce antisera (anti-N and anti-M). Both antisera stained kuru plaques in a patient with Gerstmann-Sträussler syndrome and one with CJD. Peptide-N has an amino acid sequence which does not exist in PrP27-30. Staining of kuru plaques by the antiserum against peptide-N indicated that the entire molecule, including the N-terminal portion of PrP33-35, was deposited in the kuru plaques.
MH Aged; Amino Acid Sequence; Creutzfeldt-Jakob Syndrome/immunology/pathology; Enzyme-Linked Immunosorbent Assay; Gerstmann-Sträussler-Scheinker Disease/immunology/pathology; Human; Immunoblotting; Immunohistochemistry; Kuru/immunology/*pathology; Male; Middle Age; Molecular Sequence Data; Peptides/immunology; PrPsc Proteins; Prions/*immunology
AD Department of Neurology, University of Tokyo, Japan.
SP englisch
PO Deutschland