NR AFIS
AU Herrmann,L.M.; Baszler,T.V.; Knowles,D.P.
TI PrPc mRNA, but not PrPsc is found in the salivary glands of scrapie-infected sheep
QU Biochimica et Biophysica Acta 2000 Jun 15; 1479(1-2): 147-54
PT journal article
AB Transmission studies in transmissible spongiform encephalopathies (TSEs) have become increasingly important due to the possible transmission of bovine spongiform encephalopathy to humans resulting in new variant Creutzfeldt-Jakob disease. The horizontal transmission of scrapie, a TSE of sheep, is poorly understood. Possible sources of horizontal transmission are the submandibular and parotid salivary glands. TSEs like natural sheep scrapie are characterized by the conversion of a normal protease sensitive prion protein, PrPc, to an abnormal protease resistant prion protein, PrPsc. Since the presence of PrPsc is an indicator of disease, the salivary glands of scrapie-infected sheep were examined for the presence of PrPsc. Although PrPc mRNA was detected in the salivary glands, PrPsc was not found in the salivary glands of scrapie-infected sheep. These data suggest that the salivary glands are unlikely sources of horizontal transmission of natural sheep scrapie.
MH Amino Acid Sequence; Animal; Base Sequence; Cattle; DNA Primers; Immunoblotting; Immunohistochemistry; Molecular Sequence Data; PrPc Proteins/*genetics; PrPsc Proteins/*genetics; RNA, Messenger/*genetics/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Salivary Glands/*metabolism; Scrapie/*genetics; Sheep; Support, U.S. Gov't, Non-P.H.S.
AD Animal Disease Research Unit, Agricultural Research Service, United States Department of Agriculture, Pullman, WA 99164, USA. lherrmann@vetmed.wsu.edu
SP englisch
PO Niederlande